Milton, A. published the artcileRenal tubular accumulation of organic substances: a new in vivo method which differentiates between luminal and peritubular uptake, Computed Properties of 40180-04-9, the main research area is kidney tubule uptake organic compound; radioassay kidney uptake organic compound.
By using a modification of the Sperber technique, cellular uptake of organic substances in the kidney was studied. A test substance was mixed with an extracellular marker (EDTA or inulin), both radiolabeled with an activity ratio close to 1 and injected into the renal portal system on 1 side via a leg vein. The animals were killed 1-10 min after injection and the radioactivity in different organs determined There were significantly higher ipsilateral (injection) to contralateral (control) kidney ratios (substance to marker) at 1 min after injection for Na o-[125I]iodohippurate (125I-Hipp), [14C]tetraethylammonium bromide (14C-TEA), [3H]dihydromorphine (3H-DHM), and [125I]iothalamate, with a progressive decrease in injection kidney ratios for 125I-Hipp and 14C-TEA when death occurred after a longer period. Inhibition of renal tubular transport with novobiocin or mepiperphenidol markedly reduced 1- and 4-min injection kidney ratios for 125I-Hipp and 14C-TEA, resp. When death occurred after a longer period, ratios in both kidneys increased significantly for [125I]iothalamate. A good correlation was found between peak cellular accumulation in the kidney and excretion efficiency of test substances. Thus, the results indicate (1) that 125I-Hipp, [125I]iothalamate, 14C-TEA, and 3H-DHM were accumulated from the peritubular side of the nephron through the transport systems for organic acids and bases, resp., and (2) that [125I]iothalamate also showed luminal uptake. This new in vivo technique is simple and well suited for studying renal tubular accumulation of organic substances and offers the advantage of being able to distinguish luminal from peritubular uptake.
Acta Physiologica Scandinavica published new progress about Biological transport. 40180-04-9 belongs to class benzothiophene, name is 2-(2,3-Dichloro-4-(thiophene-2-carbonyl)phenoxy)acetic acid, and the molecular formula is C13H8Cl2O4S, Computed Properties of 40180-04-9.
Referemce:
Benzothiophene – Wikipedia,
Benzothiophene | C8H6S – PubChem